Recombinant:Article Title: Glycine Substitution at Helix-to-Coil Transitions Facilitates the Structural Determination of a Stabilized Subtype C HIV Envelope Glycoprotein
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins 293fectin Invitrogen Cat#12347500 Galanthus nivalis agarose Vector Labs CatAL-1243 Endoglycosidase H New England Biolabs Cat#P0703L R-Protein A Sepharose FF GE Healthcare Cat#17127903 1086c NFL TD This paper N/A 1086c NFL TD (T536G) This paper N/A 1086c NFL TD (L537G) This paper N/A 1086c NFL TD (L544G) This paper N/A 1086c NFL TD (L568G) This paper N/A 1086c NFL TD (T569G) This paper N/A 1086c NFL TD (N636G) This paper N/A 1086c NFL TD (Y638G) This paper N/A JRFL NFL TD (T569G) This paper N/A 16055 NFL TD (T569G) This paper N/A 16055 NFL TD CC (T569G) This paper N/A 16055 NFL TD CC+ This paper N/A JRFL NFL TD CC+ This paper N/A BG505 NFL CC+ This paper N/A SC422 NFL TD CC+ This paper N/A WITO NFL TD CC+ This paper N/A 1086c NFL TD CC+ This paper N/A 001428 NFL TD CC+ This paper N/A ZM197 NFL TD CC+ This paper N/A Critical Commercial Assays QuikChange Lightning Multi Site-Directed Mutagenesis Kit Agilent Technologies Cat#210513 Deposited Data 16055 NFL TD CC (T569G), 3.9 Å structure This paper PDB: 5UM8 Experimental Models: Cell Lines Human: FreeStyle 293F cells Invitrogen Cat#R79007 Human: 293S (GnTi-) cells ATCC Cat#CRL-3022 Software and Algorithms ForteBio Data Analysis v7.1 ForteBio http://www.fortebio.com/octet- software.html Coot Emsley and Cowtan, 2004 http://www2.mrc-lmb.cam.ac.uk/personal/ pemsley/coot/ Leginon Suloway et al., 2005 http://emg.nysbc.org/redmine/projects/ leginon/wiki/Leginon_Homepage Appion Lander et al., 2009 http://emg.nysbc.org/redmine/projects/ appion/wiki/Appion_Home Clustering 2D Alignment Sorzano et al., 2010 http://xmipp.cnb.csic.es/twiki/bin/view/ Xmipp/WebHome IMAGIC van Heel et al., 1996 https://www.imagescience.de/imagic.html PHENIX Adams et al., 2010 https://www.phenix-online.org (Continued on next page) e1 Immunity 46, 792–803.e1–e3, May 16, 2017 .. REAGENT or RESOURCE SOURCE IDENTIFIER Other Anti-human IgG Fc Capture (AHC) Biosensors ForteBio Cat#18-5060 HiLoad 16/600 Superdex 200 pg column GE Healthcare Cat#28989335 Superdex 200 Increase 10/300 GL column GE Healthcare Cat#28990944 CaptureSelect LC-lambda (Hu) affinity matrix ThermoFisher Scientific Cat#084905
Mutagenesis:Article Title: Glycine Substitution at Helix-to-Coil Transitions Facilitates the Structural Determination of a Stabilized Subtype C HIV Envelope Glycoprotein
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins 293fectin Invitrogen Cat#12347500 Galanthus nivalis agarose Vector Labs CatAL-1243 Endoglycosidase H New England Biolabs Cat#P0703L R-Protein A Sepharose FF GE Healthcare Cat#17127903 1086c NFL TD This paper N/A 1086c NFL TD (T536G) This paper N/A 1086c NFL TD (L537G) This paper N/A 1086c NFL TD (L544G) This paper N/A 1086c NFL TD (L568G) This paper N/A 1086c NFL TD (T569G) This paper N/A 1086c NFL TD (N636G) This paper N/A 1086c NFL TD (Y638G) This paper N/A JRFL NFL TD (T569G) This paper N/A 16055 NFL TD (T569G) This paper N/A 16055 NFL TD CC (T569G) This paper N/A 16055 NFL TD CC+ This paper N/A JRFL NFL TD CC+ This paper N/A BG505 NFL CC+ This paper N/A SC422 NFL TD CC+ This paper N/A WITO NFL TD CC+ This paper N/A 1086c NFL TD CC+ This paper N/A 001428 NFL TD CC+ This paper N/A ZM197 NFL TD CC+ This paper N/A Critical Commercial Assays QuikChange Lightning Multi Site-Directed Mutagenesis Kit Agilent Technologies Cat#210513 Deposited Data 16055 NFL TD CC (T569G), 3.9 Å structure This paper PDB: 5UM8 Experimental Models: Cell Lines Human: FreeStyle 293F cells Invitrogen Cat#R79007 Human: 293S (GnTi-) cells ATCC Cat#CRL-3022 Software and Algorithms ForteBio Data Analysis v7.1 ForteBio http://www.fortebio.com/octet- software.html Coot Emsley and Cowtan, 2004 http://www2.mrc-lmb.cam.ac.uk/personal/ pemsley/coot/ Leginon Suloway et al., 2005 http://emg.nysbc.org/redmine/projects/ leginon/wiki/Leginon_Homepage Appion Lander et al., 2009 http://emg.nysbc.org/redmine/projects/ appion/wiki/Appion_Home Clustering 2D Alignment Sorzano et al., 2010 http://xmipp.cnb.csic.es/twiki/bin/view/ Xmipp/WebHome IMAGIC van Heel et al., 1996 https://www.imagescience.de/imagic.html PHENIX Adams et al., 2010 https://www.phenix-online.org (Continued on next page) e1 Immunity 46, 792–803.e1–e3, May 16, 2017 .. REAGENT or RESOURCE SOURCE IDENTIFIER Other Anti-human IgG Fc Capture (AHC) Biosensors ForteBio Cat#18-5060 HiLoad 16/600 Superdex 200 pg column GE Healthcare Cat#28989335 Superdex 200 Increase 10/300 GL column GE Healthcare Cat#28990944 CaptureSelect LC-lambda (Hu) affinity matrix ThermoFisher Scientific Cat#084905
Software:Article Title: Glycine Substitution at Helix-to-Coil Transitions Facilitates the Structural Determination of a Stabilized Subtype C HIV Envelope Glycoprotein
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins 293fectin Invitrogen Cat#12347500 Galanthus nivalis agarose Vector Labs CatAL-1243 Endoglycosidase H New England Biolabs Cat#P0703L R-Protein A Sepharose FF GE Healthcare Cat#17127903 1086c NFL TD This paper N/A 1086c NFL TD (T536G) This paper N/A 1086c NFL TD (L537G) This paper N/A 1086c NFL TD (L544G) This paper N/A 1086c NFL TD (L568G) This paper N/A 1086c NFL TD (T569G) This paper N/A 1086c NFL TD (N636G) This paper N/A 1086c NFL TD (Y638G) This paper N/A JRFL NFL TD (T569G) This paper N/A 16055 NFL TD (T569G) This paper N/A 16055 NFL TD CC (T569G) This paper N/A 16055 NFL TD CC+ This paper N/A JRFL NFL TD CC+ This paper N/A BG505 NFL CC+ This paper N/A SC422 NFL TD CC+ This paper N/A WITO NFL TD CC+ This paper N/A 1086c NFL TD CC+ This paper N/A 001428 NFL TD CC+ This paper N/A ZM197 NFL TD CC+ This paper N/A Critical Commercial Assays QuikChange Lightning Multi Site-Directed Mutagenesis Kit Agilent Technologies Cat#210513 Deposited Data 16055 NFL TD CC (T569G), 3.9 Å structure This paper PDB: 5UM8 Experimental Models: Cell Lines Human: FreeStyle 293F cells Invitrogen Cat#R79007 Human: 293S (GnTi-) cells ATCC Cat#CRL-3022 Software and Algorithms ForteBio Data Analysis v7.1 ForteBio http://www.fortebio.com/octet- software.html Coot Emsley and Cowtan, 2004 http://www2.mrc-lmb.cam.ac.uk/personal/ pemsley/coot/ Leginon Suloway et al., 2005 http://emg.nysbc.org/redmine/projects/ leginon/wiki/Leginon_Homepage Appion Lander et al., 2009 http://emg.nysbc.org/redmine/projects/ appion/wiki/Appion_Home Clustering 2D Alignment Sorzano et al., 2010 http://xmipp.cnb.csic.es/twiki/bin/view/ Xmipp/WebHome IMAGIC van Heel et al., 1996 https://www.imagescience.de/imagic.html PHENIX Adams et al., 2010 https://www.phenix-online.org (Continued on next page) e1 Immunity 46, 792–803.e1–e3, May 16, 2017 .. REAGENT or RESOURCE SOURCE IDENTIFIER Other Anti-human IgG Fc Capture (AHC) Biosensors ForteBio Cat#18-5060 HiLoad 16/600 Superdex 200 pg column GE Healthcare Cat#28989335 Superdex 200 Increase 10/300 GL column GE Healthcare Cat#28990944 CaptureSelect LC-lambda (Hu) affinity matrix ThermoFisher Scientific Cat#084905
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